Spirulina Powder

SPIRULINA POWDER
(Certified Organic) Spray Dried

Botanical Name: Arthrospira platensis

Product Code: THO2045

HS Code: 12122990

TEST STANDARD

Analysis

Description

Test Method

Macroscopic Characteristics

Fine uniform blue green to green with mild smell and test

Visual/Organoleptic Test

Microscopic Characteristics

6-10 micron in Diameter
20-60 micron in Length

Spirulina Monograph, (USP)

Particle size (100 mesh)

Min 85% pass through

USP, Chapter (786)

Loss on Drying,%

Max. 9.0

USP, Chapter (781)

Total Ash (including Minerals) %

Max. 9.0

USP, Chapter (561)

Acid Insoluble Ash

Max. 0.50

USP, Chapter (561)

Bulk Density (Tapped), g/ml

0.65-0.85

IS 1462-1985

Protein %

63.0-68.0

USP, Chapter 461, Method I Nitrates and Nitrites Present

β Carotene, mg/100g

Min 160.0

IS 5886 -1970

Chlorophyll- a,%

Min. 1.0

AOAC (1995):940.03

Phycocyanin,%

Min 10.0

Bausssiba S. and Richmond 1979. Isolation and Purification from blue alga Spirulina platensis. Arch. Microbial. 120: 155-159

MICROBIOLOGICAL ANALYSIS

Analysis

Limit/Specification

Test Method

Total aerobic count cfu/g

Not more than 50000

USP Chapter (2021)

Yeasts & Molds counts cfu/g

Not more than 100

USP Chapter (2021)

E.coli per g

Absent

USP Chapter (2022)

Coliforms per 0.1 g

Absent

USP Chapter (2022)

Salmonella sp. per g

Absent

USP Chapter (2022)

Staphylococcus per g

Absent

USP Chapter (2022)

PESTICIDES

Analysis

Limit/Specification

Test Method

Pesticides

As per USDA NOP standards

AOAC 2007.01

HEAVY METAL

Analysis

Detection Limit

Test Method

Lead

NMT 3.0 mg/kg

ICP - MS

Cadmium

NMT 1.0 mg/kg

ICP - MS

Mercury

NMT  0.01 mg/kg

ICP - MS

ADDITIONAL TEST

Analysis

Detection Limit

Test Method

Bile tolerant gram negative CFU/G

NMT 100

USP, Chapter (2021)

PAH, µg/kg

30

GC – FID

PCB, mg/kg

0.1

GCMS

AFLATOXIN (B1, B2, G1 & G2), ppb

0.5-1.0

LCMS/MS

FATTY ACID PROFILE

Analysis

Detection Limit

Test Method

Palmitic acid, %

35-60

By GC, Spirulina Monograph, USP & System Suitability(621)

Palmitoleic acid, %

2-8

Stearic acid, %

1-5

Oleic acid, %

1-7

Linoleic acid, %

13-25

Gama linoleic acid, %

13-27

Alpha linoleic acid,%

< 0.5


STORAGE CONDITION

Store in a cool and dry place, away from sunlight. Do not leave in an open dry to avoid external contamination and preventing nutrient deterioration.

Shelf Life

36 months from the date of manufacture

SPIRULINA POWDER(Certified Organic) Spray Dried Botanical Name: Arthrospira platensis Product Code: THO2045 HS Code: 12122990

TEST STANDARD

Analysis Macroscopic Characteristics
Description Fine uniform blue green to green with mild smell and test.
Test Method Visual/Organoleptic Test
Analysis Microscopic Characteristics
Description 6-10 micron in Diameter
20-60 micron in Length
Test Method Spirulina Monograph, (USP)
Analysis Particle size (100 mesh)
Description Min 85% pass through
Test Method USP, Chapter (786)
Analysis Loss on Drying,%
Description Max. 9.0
Test Method USP, Chapter (781)
Analysis Total Ash (including Minerals) %
Description Max. 9.0
Test Method USP, Chapter (561)
Analysis Acid Insoluble Ash
Description Max. 0.50
Test Method USP, Chapter (561)
Analysis Bulk Density (Tapped), g/ml
Description 0.65-0.85
Test Method IS 1462-1985
Analysis Protein %
Description 63.0-68.0
Test Method USP, Chapter 461, Method I Nitrates and Nitrites Present
Analysis β Carotene, mg/100g
Description Min 160.0
Test Method IS 5886 -1970
Analysis Chlorophyll- a,%
Description Min. 1.0
Test Method AOAC (1995):940.03
Analysis Phycocyanin,%
Description Min 10.0
Test Method Bausssiba S. and Richmond 1979. Isolation and Purification from blue alga Spirulina platensis. Arch. Microbial. 120: 155-159

MICROBIOLOGICAL ANALYSIS

Analysis Total plate count
Limit/Specification Not more than 50000
Test Method USP Chapter (2021)
Analysis Yeasts & Molds
Limit/Specification Not more than 100
Test Method USP Chapter (2021)
Analysis E.coli per g
Limit/Specification Absent
Test Method USP Chapter (2022)
Analysis Coliforms per 0.1 g
Limit/Specification Absent
Test Method USP Chapter (2022)
Analysis Salmonella sp. per g
Limit/Specification Absent
Test Method USP Chapter (2022)
Analysis Staphylococcus per g
Limit/Specification Absent
Test Method USP Chapter (2022)

PESTICIDES

Analysis Pesticides
Limit/Specification As per USDA NOP standards
Test Method AOAC 2007.01

HEAVY METAL

Analysis Lead
Limit/Specification NMT 3.0 mg/kg
Test Method ICP – MS
Analysis Cadmium
Limit/Specification NMT 1.0 mg/kg
Test Method ICP – MS
Analysis Mercury
Limit/Specification NMT 0.01 mg/kg
Test Method ICP – MS

ADDITIONAL TEST

Analysis Bile tolerant gram negative CFU/G
Detection Limit NMT 100
Test Method USP, Chapter (2021)
Analysis PAH, µg/kg
Detection Limit 30
Test Method GC – FID
Analysis PCB, mg/kg
Detection Limit 0.1
Test Method GCMS
Analysis AFLATOXIN (B1, B2, G1 & G2), ppb
Detection Limit 0.5-1.0
Test Method LCMS/MS

FATTY ACID PROFILE

Analysis Palmitic acid, %
Detection Limit 35-60
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Palmitoleic acid, %
Detection Limit 2-8
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Stearic acid, %
Detection Limit 1-5
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Oleic acid, %
Detection Limit 1-7
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Linoleic acid, %
Detection Limit 13-25
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Gama linoleic acid, %
Detection Limit 13-27
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
Analysis Alpha linoleic acid,%
Detection Limit < 0.5
Test Method By GC, Spirulina Monograph, USP & System Suitability(621)
STORAGE CONDITION Store in a cool and dry place, away from sunlight. Do not leave in an open dry to avoid external contamination and preventing nutrient deterioration. Shelf Life 36 months from date of manufacture